Analyzing malware with API calls plays a huge role in malware analysis. Thus, APIs can give malware analysts an idea about malware behavior, especially when basic, static analysis wasn't successful due to obfuscation techniques (like packers, crypters, and protectors). Malware analysts can gain an understanding of how a malicious file works by studying API calls. There are many online tools that will give you the ability to analyze malware in a secure environment. Those utilities and environments are called sandboxes. Malware that is detected is identified by a hash function (MD5 or SHA256). Malware analysts use hashing to sign a file. For example, the following APIs were taken from the report of an online malware scan with https://www.hybrid-analysis.com.
These are some details about the malware "PE32 executable (GUI...