Correcting images
In order to analyze images, we sometimes need to correct the problems that were present during acquisition. Problems such as noise, uneven illumination, and background fluorescence can cause many issues during image analysis. I will provide a little technical background on the sources of these problems and then follow this up with how they can be corrected in ImageJ.
Technical background
Of the many sources of noise that exist in imaging, a few can be corrected with correct acquisition settings. Others are inherent in the electronics and physical properties of the camera, and cannot be easily fixed. I will first deal with the source of noise that can be remedied with optimizing acquisition: Shot or Poisson noise. Next, we will look at Electronic or Dark noise.
Correcting Shot noise
Shot noise is caused by the physical properties of light; light can be seen as packages of light or photons. The number of photons that are collected by each photo-detector site on the camera determines...
