Colocalization

In the previous sections, we looked at ways to measure certain aspects of our images, such as velocity and length. This section will look at a different aspect of measurements that involve the colocalization of different signals. Colocalization means that two (or more) objects are within proximity of each other. Whenever two signals overlap in space, we can conclude that they are at the same location, within the boundaries of the resolution that an imaging system allows us. In biology, the localization of two labeled structures or proteins provides clues to whether the protein is contained within a structure or whether it moves to a certain location after being stimulated. For dynamic structures such as cells, we can observe a change in the amount of colocalization, depending on time or stimulation.

Semiquantitative colocalization

Semiquantitative colocalization means that you examine the amount of colocalization by eye or using a crude measurement and classify it (rather arbitrarily...