Chapter 3. Advanced Image Processing with ImageJ
In the previous chapter, we learned how to read images, the types of images that we can handle, and how to manipulate the intensity levels in order to improve visualization or segment part of the pixels depending on their intensity values. Now we will extend our abilities to measure and manipulate our images. First we will learn what are selections, regions of interest, and overlays, and how to work with them and what can be measured using these tools. After this, we will focus on some classic image processing both in spatial and frequency domains, understanding what that means. Finally, we will see how particle analysis can be done in ImageJ.